ASTM F2944 Standard Test Method for Automated Colony Forming Unit (CFU) Assays-Image Acquisition and Analysis Method for Enumerating and Characterizing Cells and Colonies in Culture
Данный раздел/документ содержится в продуктах:
- Техэксперт: Машиностроительный комплекс
- Картотека зарубежных и международных стандартов
- ASTM E2402 Standard Test Method for Mass Loss and Residue Measurement Validation of Thermogravimetric Analyzers
- ASTM E1970 Standard Practice for Statistical Treatment of Thermoanalytical Data
- ASTM F1469 Standard Guide for Conducting a Repeatability and Reproducibility Study on Test Equipment for Nondestructive Testing
- ASTM E1970 Standard Practice for Statistical Treatment of Thermoanalytical Data
- ASTM E2918 Standard Test Method for Performance Validation of Thermomechanical Analyzers
- ASTM E1970 Standard Practice for Statistical Treatment of Thermoanalytical Data
- ASTM F1469 Standard Guide for Conducting a Repeatability and Reproducibility Study on Test Equipment for Nondestructive Testing
- ASTM E1970 Standard Practice for Statistical Treatment of Thermoanalytical Data
- ASTM E2161 Standard Terminology Relating to Performance Validation in Thermal Analysis
- ASTM E2918 Standard Test Method for Performance Validation of Thermomechanical Analyzers
- ASTM E1970 Standard Practice for Statistical Treatment of Thermoanalytical Data
- ASTM F1469 Standard Guide for Conducting a Repeatability and Reproducibility Study on Test Equipment for Nondestructive Testing
- ASTM E1970 Standard Practice for Statistical Treatment of Thermoanalytical Data
- ASTM E2918 Standard Test Method for Performance Validation of Thermomechanical Analyzers
- 01
- ASTM E2161 Standard Terminology Relating to Performance Validation in Thermal Analysis
- ASTM E2918 Standard Test Method for Performance Validation of Thermomechanical Analyzers
- ASTM E1970 Standard Practice for Statistical Treatment of Thermoanalytical Data
- ASTM F1469 Standard Guide for Conducting a Repeatability and Reproducibility Study on Test Equipment for Nondestructive Testing
- ASTM E1970 Standard Practice for Statistical Treatment of Thermoanalytical Data
- ASTM E2918 Standard Test Method for Performance Validation of Thermomechanical Analyzers
- 01.040
- ASTM E2161 Standard Terminology Relating to Performance Validation in Thermal Analysis
- ASTM E2918 Standard Test Method for Performance Validation of Thermomechanical Analyzers
- ASTM E1970 Standard Practice for Statistical Treatment of Thermoanalytical Data
- ASTM F1469 Standard Guide for Conducting a Repeatability and Reproducibility Study on Test Equipment for Nondestructive Testing
- ASTM E1970 Standard Practice for Statistical Treatment of Thermoanalytical Data
- ASTM E2918 Standard Test Method for Performance Validation of Thermomechanical Analyzers
- ASTM E2161 Standard Terminology Relating to Performance Validation in Thermal Analysis
- ASTM E2161 Standard Terminology Relating to Performance Validation in Thermal Analysis
- ASTM E1970 Standard Practice for Statistical Treatment of Thermoanalytical Data
- ASTM F1469 Standard Guide for Conducting a Repeatability and Reproducibility Study on Test Equipment for Nondestructive Testing
- ASTM F1469 Standard Guide for Conducting a Repeatability and Reproducibility Study on Test Equipment for Nondestructive Testing
- ASTM F2944 Standard Test Method for Automated Colony Forming Unit (CFU) Assays-Image Acquisition and Analysis Method for Enumerating and Characterizing Cells and Colonies in Culture
- ASTM F2149 Standard Test Method for Automated Analyses of Cells - the Electrical Sensing Zone Method of Enumerating and Sizing Single Cell Suspensions
- ASTM E2402 Standard Test Method for Mass Loss and Residue Measurement Validation of Thermogravimetric Analyzers
- Картотека зарубежных и международных стандартов
ASTM International
Standard Test Method for Automated Colony Forming Unit (CFU) Assays-Image Acquisition and Analysis Method for Enumerating and Characterizing Cells and Colonies in Culture
N F2944
Annotation
This test method, provided its limitations are understood, describes a procedure for quantitative measurement of the number and biological characteristics of colonies derived from a stem cell or progenitor population using image analysis.
This test method is applied in an in vitro laboratory setting.
This method utilizes: (a) standardized protocols for image capture of cells and colonies derived from in vitro processing of a defined population of starting cells in a defined field of view (FOV), and (b) standardized protocols for image processing and analysis.
The relevant FOV may be two-dimensional or threedimensional, depending on the CFU assay system being interrogated.
The primary unit to be used in the outcome of analysis is the number of colonies present in the FOV. In addition, the characteristics and sub-classification of individual colonies and cells within the FOV may also be evaluated, based on extant morphological features, distributional properties, or properties elicited using secondary markers (for example, staining or labeling methods).
Imaging methods require that images of the relevant FOV be captured at sufficient resolution to enable detection and characterization of individual cells and over a FOV that is sufficient to detect, discriminate between, and characterize colonies as complete objects for assessment.
Image processing procedures applicable to two- and three-dimensional data sets are used to identify cells or colonies as discreet objects within the FOV. Imaging methods may be optimized for multiple cell types and cell features using analytical tools for segmentation and clustering to define groups of cells related to each other by proximity or morphology in a manner that is indicative of a shared lineage relationship (that is, clonal expansion of a single founding stem cell or progenitor).
