ASTM F2998 Guide for Using Fluorescence Microscopy to Quantify the Spread Area of Fixed Cells
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- Техэксперт: Машиностроительный комплекс
- Картотека зарубежных и международных стандартов
- ASTM E2402 Standard Test Method for Mass Loss and Residue Measurement Validation of Thermogravimetric Analyzers
- ASTM E1970 Standard Practice for Statistical Treatment of Thermoanalytical Data
- ASTM F1469 Standard Guide for Conducting a Repeatability and Reproducibility Study on Test Equipment for Nondestructive Testing
- ASTM E1970 Standard Practice for Statistical Treatment of Thermoanalytical Data
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- ASTM E1970 Standard Practice for Statistical Treatment of Thermoanalytical Data
- ASTM F1469 Standard Guide for Conducting a Repeatability and Reproducibility Study on Test Equipment for Nondestructive Testing
- ASTM E1970 Standard Practice for Statistical Treatment of Thermoanalytical Data
- ASTM E2161 Standard Terminology Relating to Performance Validation in Thermal Analysis
- ASTM E2918 Standard Test Method for Performance Validation of Thermomechanical Analyzers
- ASTM E1970 Standard Practice for Statistical Treatment of Thermoanalytical Data
- ASTM F1469 Standard Guide for Conducting a Repeatability and Reproducibility Study on Test Equipment for Nondestructive Testing
- ASTM E1970 Standard Practice for Statistical Treatment of Thermoanalytical Data
- ASTM E2918 Standard Test Method for Performance Validation of Thermomechanical Analyzers
- 01
- ASTM E2161 Standard Terminology Relating to Performance Validation in Thermal Analysis
- ASTM E2918 Standard Test Method for Performance Validation of Thermomechanical Analyzers
- ASTM E1970 Standard Practice for Statistical Treatment of Thermoanalytical Data
- ASTM F1469 Standard Guide for Conducting a Repeatability and Reproducibility Study on Test Equipment for Nondestructive Testing
- ASTM E1970 Standard Practice for Statistical Treatment of Thermoanalytical Data
- ASTM E2918 Standard Test Method for Performance Validation of Thermomechanical Analyzers
- 01.040
- ASTM E2161 Standard Terminology Relating to Performance Validation in Thermal Analysis
- ASTM E2918 Standard Test Method for Performance Validation of Thermomechanical Analyzers
- ASTM E1970 Standard Practice for Statistical Treatment of Thermoanalytical Data
- ASTM F1469 Standard Guide for Conducting a Repeatability and Reproducibility Study on Test Equipment for Nondestructive Testing
- ASTM E1970 Standard Practice for Statistical Treatment of Thermoanalytical Data
- ASTM E2918 Standard Test Method for Performance Validation of Thermomechanical Analyzers
- ASTM E2161 Standard Terminology Relating to Performance Validation in Thermal Analysis
- ASTM E2161 Standard Terminology Relating to Performance Validation in Thermal Analysis
- ASTM E1970 Standard Practice for Statistical Treatment of Thermoanalytical Data
- ASTM F1469 Standard Guide for Conducting a Repeatability and Reproducibility Study on Test Equipment for Nondestructive Testing
- ASTM F1469 Standard Guide for Conducting a Repeatability and Reproducibility Study on Test Equipment for Nondestructive Testing
- ASTM F2944 Standard Test Method for Automated Colony Forming Unit (CFU) Assays-Image Acquisition and Analysis Method for Enumerating and Characterizing Cells and Colonies in Culture
- ASTM F2149 Standard Test Method for Automated Analyses of Cells - the Electrical Sensing Zone Method of Enumerating and Sizing Single Cell Suspensions
- ASTM F2739 Standard Guide for Quantifying Cell Viability within Biomaterial Scaffolds
- ASTM E2402 Standard Test Method for Mass Loss and Residue Measurement Validation of Thermogravimetric Analyzers
- Картотека зарубежных и международных стандартов
ASTM International
Guide for Using Fluorescence Microscopy to Quantify the Spread Area of Fixed Cells
N F2998
Annotation
This guide describes several measurement and technical issues involved in quantifying the spread area of fixed cells. Cell spreading and the distribution of cell spread areas of a population of cells are the result of a biological response that is dependent on intracellular signaling mechanisms and the characteristics of cell adhesion to a surface. Cell spread area is a morphological feature that can be responsive to alteration in the metabolic state or the state of stress of the cells. Changes in cell spread area can also indicate an alteration in the adhesion substrate that may be due to differences in manufacturing of the substrate material or be in response to extracellular matrix secretions. High quality measurement of cell spread area can serve as a useful metric for benchmarking and detecting changes cell behavior under experimental conditions.
The measurement described in this document is based on the use of fluorescence microscopy imaging of fixed cells and the use of image analysis algorithms to extract relevant data from the images. To produce robust cell spread area measurements, technical details involved in sample preparation, cell staining, microscopy imaging, image analysis and statistical analysis should be considered. Several of these issues are discussed within this document.
This standard is meant to serve as a guide for developing methods to reliably measure the area to which cells spread at a surface. This surface can be conventional tissue culture polystyrene or sophisticated engineered biomaterial surfaces. An example of a detailed procedure to measure the spreading area of cells on a tissue culture polystyrene surface is provided in the appendix section.
Cell morphology features such as cell spreading area and perimeter are generally reported in units of length. For example, spreading area per cell (that is, cell spread area) is likely reported in units of m2. A spatial calibration standard is required to convert between numbers of pixels in a CCD camera image to m2 as an SI unit.
