ASTM International

Standard Test Method for Evaluating Disinfectant Efficacy Against Pseudomonas aeruginosa Biofilm Grown in CDC Biofilm Reactor Using Single Tube Method
 N E2871

Annotation

This test method specifies the operational parameters required to perform a quantitative liquid disinfectant efficacy test against biofilm bacteria.

The test method was developed using a Pseudomonas aeruginosa biofilm grown in the CDC Biofilm Reactor (Test Method E2562), modified to include borosilicate glass coupons as a hard nonporous surface and P. aeruginosa ATCC 15442.

Disinfectant preparation and contact time are used in the assessment according to the manufacturer's instructions for use.

The test method uses a closed system to treat biofilm. A coupon is placed in a single tube for the treatment, neutralization, and sampling steps to prevent the loss of cells.

Verification of disinfectant neutralization is determined prior to conducting the test method.

This test method describes how to sample and analyze treated and untreated control biofilms for viable cells. Biofilm population density is recorded as log10 colony-forming units per coupon. Efficacy is reported as a log10 reduction of viable cells.

Basic microbiology training is required to perform this assay.

The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.