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ASTM E1604 Standard Guide for Behavioral Testing in Aquatic Toxicology

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ASTM International

Standard Guide for Behavioral Testing in Aquatic Toxicology
 N E1604

 

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This guide covers some general information on the selection and application of behavioral methods useful for determining the sublethal effects of chemicals to fish, amphibians, and macroinvertebrates.

Behavioral toxicity occurs when chemical or other stressful conditions, such as changes in water quality or temperature, induce a behavioral change that exceeds the normal range of variability (1).2 Behavior includes all observable, recordable, or measurable activities of a living organism and reflects genetic, neurobiological, physiological, and environmental determinants (2).

Behavioral methods can be used in biomonitoring, the determination of no-observed-effect and lowest-observedeffect concentrations, and the prediction of hazardous chemical impacts on natural populations (3).

Behavioral methods can be applied to fish, amphibians, and macroinvertebrates in standard laboratory toxicity tests, tests of effluents, and sediment toxicity tests.

The various behavioral methods included in this guide are categorized with respect to seven interdependent, functional responses that fish, amphibians, and macroinvertebrates must perform in order to survive. These functional responses include respiration, locomotion, habitat selection, feeding, predator avoidance, competition, and reproduction (4). These responses can be documented visually or through video or acoustic imagery. Electronically recorded information can be derived through manual techniques or through the use of digital image analysis software. (5, 6, 7)

The functional responses are not necessarily mutually exclusive categories. For instance, locomotion, of some form of movement, is important to all behavioral functions.

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