ASTM International

Standard Guide for Conducting Renewal Phytotoxicity Tests With Freshwater Emergent Macrophytes
 N E1841

Annotation

This test guide is designed to give general guidance for assessing the potential phytotoxicity of water soluble test material to freshwater emergent macrophytes.

This renewal test continuously exposes selected plant species, growing in sediment, to various concentrations of test material, dissolved in a nutrient solution.

This test guide is based on the Toxic Substances Control Act (TSCA) guidelines for conducting toxicity tests with terrestrial plants (1)2 and is applicable to most water soluble chemicals, either individually or in formulations, commercial products, or known mixtures (see Guides E1193 and E1598). With slight modifications the procedure also might be used for effluents (see Guide E1192).

Results from this toxicity test can be used to report an IC50 or NOEC (see Section 3) based on the concentration of chlorophyll extracted from the plants (see Guides D3731 and E1218). In some situations, it might be necessary to only test at one concentration to determine whether or not that specific concentration is toxic to the plants.

The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Specific hazard statements are given in Section 7.

2 The boldface numbers in parentheses refer to the list of references at the end of this test method.